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Maghemite Nanoparticle Induced DNA Damage and Oxidative Stress Mediated Apoptosis of CRL-1739 Adenogastric Carcinoma Cell

[ Vol. 8 , Issue. 1 ]

Author(s):

Karthikeyan Manikandan, Amutha Santhanam* and Setty Balakrishnan Anand   Pages 58 - 68 ( 11 )

Abstract:


Background: Maghemite nanoparticle is well known for its biocompatibility compared to other types of nanoparticles having huge biomedical applications. The aim of this study was to investigate the significance of maghemite nanoparticle and analyse the anti proliferative, oxidative stress and apoptosis potency of this on CRL-1739 Adenogastric carcinoma (AGS) cell line in order to understand its antitumor effect on gastric carcinoma.

Methods: The cytotoxicity was examined by MTT assay and microscopic analysis on AGS cell line along with a small intestinal epithelial cell line IEC-6. The oxidative stress and cell damage on AGS were monitored by measuring the anti oxidants of glutathione S Transferase (GST), reduced glutathione (GSH) and trypan blue exclusion and Lactate dehydrogenase release (LDH) assay respectively. In addition live/dead assay, DNA fragmentation assay and semi quantitative PCR analysis were carried out.

Results: The cytotoxicity results showed that significant growth inhibition was observed in the cancer cells compared to normal cells. The cytotoxicity resulted here was due to oxidative stress that reflected in the reduction of antioxidants and elevated levels of ROS and LDH. Moreover, it was investigated that nanoparticles arrested cell growth in the G2/M phase transition. The cell death mechanism induced by the nanoparticle upregulated the expression of apoptotic marker p53 along with down regulation of oncogene C-myc.

Conclusion: The result obtained in this study suggests that the maghemite nanoparticle explode the activation of apoptosis in cancer cells by G2/M phase arrest as well as shows to be a potent targeted therapeutic agent.

Keywords:

Adenocarcinoma, anti-proliferative, apoptosis, G2/M phase, maghemite nanoparticle, normal cells IEC-6.

Affiliation:

Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, National Centre for Nanoscience and Nanotechnology, University of Madras, Guindy Campus, Chennai, Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai

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