Himangi Kayath, Surbhi Dhawan and Sanju Nanda* Pages 216 - 231 ( 16 )
Background: The use of natural actives for cosmeceutical benefits has recently gained high popularity due to their low side effects, natural origin and safety. The fruits of Rosehip, a wild shrub native to Europe, Northwest Africa, and Western Asia have been used in traditional medicines since ancient times. Seeds of Rosehip are known to contain more than 50% of polyunsaturated fatty acids. Inherently, this class of phytoconstituents has been reported to exhibit anti-aging potential.Objective: The objective of the present study was to explore and establish the photoprotective potential of Rosehip Seed Oil (RSO) obtained from Rosa rubigenosa and develop a QbD-based topical nanoformulation. Methods: The photo-protective potential of Rosehip Seed Oil was evaluated through various in-vitro tests and biochemical assays. The o/w nanoemulsion (NE) of the oil was prepared by the ultrasonication method following Central Composite Design (CCD) to find the best possible formulation conditions. Nanoemulsion was further formulated into a topical gel and evaluated for its semisolid attributes. Results: The antioxidant property of Rosehip Seed Oil assessed by estimating DPPH free radical scavenging activity revealed that 45μg/ml of oil was equivalent to 8μg/ml of α- tocopherol (standard). The total phenolic content of the oil was estimated to be 5.81μg/ml. IC50 values for anti-collagenase, anti-elastase and anti-hyaluronidase activities were determined and found to be 7.34mg/ml, 42.3mg/ml and 36.34mg/ml, respectively. The particle size, PDI, zeta potential, viscosity and pH of the optimized batch (R2) were found to be 82.73nm, 0.214, -23.0 mV, 6.65cp and 6.94, respectively. The cumulative release of the drug from the nanoemulsion gel was estimated for 7 hrs and found to be 72.34%. Conclusion: Results of all the in vitro studies performed led to the conclusion that Rosehip oil possesses very good antioxidant, anti-inflammatory and photo-protective properties and a nanoformulation of this plant seed oil has the potential to help in assuaging the detrimental effects of UV rays like photoaging.
Rosehip seed oil, photo-protection, anti-oxidant, biochemical, central composite design, nanoemulsion, ultrasonication.
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana, Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana, Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak-124001, Haryana